To assess the function of introduced Ii, flow cytometry was used to measure the levels (histogram and median MFI) of I-Ag7, the MHC class II allele expressed by NOD mice. I-Ag7 levels were compared between the Flag+ (representing cells expressing exogenous Ii) and Flag? populations (representing non-expressing, likely non-transduced, cells) in recipients of BM-HSC transduced by WT or mutant Ii con


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